Human skin test antigen stimulated killer (STAK) cells generated in vitro are Leu-11b positive and nonadherent to plastic
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6859600
Human peripheral blood mononuclear cells were exposed to solubilized skin test antigen (STA) in vitro for 5 days. After culture natural effector cell activity was determined in the STAK cell populations after no treatment (N.T.), after treatment with Leu-11b mouse monoclonal antibody plus rabbit complement and after incubation on tissue culture grade plastic for one hour at 37/sup 0/C to remove cells adherent to plastic. Natural cytotoxicity in the STAK cell populations after treatment was determined in 4 hr /sup 51/Cr release assays using /sup 51/Cr labeled K562 target cells. The results show that STAK cells belong to the subset of mononuclear natural effector cells that do not appear to be in the monocyte lineage since they possess the Leu-11b surface antigen and they are nonadherent to plastic.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 6859600
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHEMICAL COMPOSITION
CHROMIUM 51
CHROMIUM ISOTOPES
COMPLEMENT
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ORGANS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
PRIMATES
RABBITS
RADIOISOTOPES
RODENTS
SKIN
SOMATIC CELLS
SYNTHETIC MATERIALS
TISSUE CULTURES
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHEMICAL COMPOSITION
CHROMIUM 51
CHROMIUM ISOTOPES
COMPLEMENT
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ORGANS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
PRIMATES
RABBITS
RADIOISOTOPES
RODENTS
SKIN
SOMATIC CELLS
SYNTHETIC MATERIALS
TISSUE CULTURES
TRACER TECHNIQUES
VERTEBRATES