Demonstration of tumor-associated antigens in dogs

Journal Article · Mon Dec 01 04:00:00 EST 1975 · Transplant. Proc., v. 7, no. 4, pp. 481-484

OSTI ID:4118410

Warren, R P; Tsoi, M S; Henderson, B H; Weiden, P L; Storb, R

Procedures for the $sup 51$Cr release assay using labeled L cells or lymphocytes and the indirect leukocyte migration test are described. These assays appear capable of detecting cellular immunity against tumor associated antigens in dogs. In 8 of 14 instances, lymphocyte-mediated cytotoxicity to autochthonous L cells was found as measured by $sup 51$Cr release, and lymphocytes from 12 of 24 dogs produced the migration inhibition factor (MIF) when cultured with autochthonous tumor cells. Chromium-51 release occurred with 4 h of exposure of effector cells to target cells, suggesting that the immunity detected in the tumor dogs against their tumors is also present in vivo. With the leukocyte migration test, however, consistent MIF production was achieved only after 3 to 6 days in culture, suggesting that in vitro sensitization may result in increased MIF production. (HLW)

• Research Organization: Univ. of Washington, Seattle
• NSA Number: NSA-33-012547
• OSTI ID: 4118410
• Journal Information: Transplant. Proc., v. 7, no. 4, pp. 481-484, Journal Name: Transplant. Proc., v. 7, no. 4, pp. 481-484; ISSN TICPB
• Country of Publication: United States
• Language: English

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*ANTIGENS-- IMMUNITY
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