In vitro interactions of lymphocytes and cultured cells from beagles with plutonium-induced bone tumors

Conference · Thu Jan 01 04:00:00 EST 1976

OSTI ID:4052568

Frazier, M E; Lund, J E; Busch, R H

Cell cultures have been prepared from lung and bone tumors arising in beagle dogs following exposure to inhaled plutonium. Evaluation of the cultured cells by commonly applied criteria (i.e., cell morphology, lack of contact inhibitory mechanisms, cloning efficiency, growth in soft agar, and tumor production in vivo) indicated that tumor cells were being grown in culture. Blood leukocytes and peripheral lymphocytes from beagle dogs were tested for cytotoxic effects against several cell cultures. Lymphocytes from normal dogs or dogs with unrelated tumors would not kill the bone tumor cells unless monocytes (macrophage) were present, in which case the leukocyte preparation was capable of mounting de novo cytotoxic immune reactions after 3 to 5 days in culture. In contrast, the dogs with plutonium-induced bone tumors had circulating lymphocytes that appeared to have undergone presensitization to bone-tumor-distinctive antigens in vivo. Consequently these lymphocytes interacted with cultured cells promptly after encounter in vitro. (auth)

• Research Organization: Battelle Pacific Northwest Labs., Richland, WA; Battelle Pacific Northwest Labs., Richland, Wash. (USA); USAEC Division of Biomedical and Environmental Research, Washington, D.C.
• NSA Number: NSA-33-023566
• OSTI ID: 4052568
• Report Number(s): CONF-740930--
• Country of Publication: United States
• Language: English

Related Subjects

*LYMPHOCYTES-- IMMUNE REACTIONS
560162* --Biomedical Sciences
Applied Studies--Radiation Effects--Radionuclide Effects--Internal Source--Animals
BEAGLES
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
N48620* --Life Sciences--Radionuclide Effects (Internal Source)--Animals
OSTEOSARCOMAS
PLUTONIUM
RADIOINDUCTION
SKELETON