In vitro formation of osteoclasts from long-term cultures of bone marrow mononuclear phagocytes

Journal Article · Wed Dec 01 04:00:00 EST 1982 · J. Exp. Med.; (United States)

DOI:https://doi.org/10.1084/jem.156.6.1604· OSTI ID:6145998

Burger, E H; Van der Meer, J W; van de Gevel, J S; Gribnau, J C; Thesingh, G W; van Furth, R

The origin of osteoclasts was studied in an in vitro model using organ cultures of periosteum-free embryonic mouse long-bone primordia, which were co-cultured with various cell populations. The bone rudiments were freed of their periosteum-perichondrium by collagenase treatment in a stage before cartilage erosion and osteoclast formation, and co-cultured for 7 d with either embryonic liver or mononuclear phagocytes from various sources. Light and electron microscopic examination of the cultures showed that mineralized matrix-resorbing osteoclasts developed only in bones co-cultured with embryonic liver or with cultured bone marrow mononuclear phagocytes but not when co-cultured with blood monocytes or resident or exudate peritoneal macrophages. Osteoclasts developed from the weakly adherent, but not from the strongly adherent cells of bone marrow cultures, whereas 1,000 rad irradiation destroyed the capacity of such cultures to form osteoclasts. In bone cultures to which no other cells were added, osteoclasts were virtually absent. Bone-resorbing activity of in vitro formed osteoclasts was demonstrated by /sup 45/Ca release studies. These studies demonstrate that osteoclasts develop from cells present in cultures of proliferating mononuclear phagocytes and that, at least in our system, monocytes and macrophages are unable to form osteoclasts. The most likely candidates for osteoclast precursor cells seem to be monoblasts and promonocytes.

• Research Organization: Department of Cell Biology and Histology, University Hospital, Leiden, Netherlands
• OSTI ID: 6145998
• Journal Information: J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 156:6; ISSN JEMEA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE CELLS
BONE MARROW CELLS
CALCIUM 45
CALCIUM ISOTOPES
CELL CULTURES
CHEMISTRY
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DAYS LIVING RADIOISOTOPES
DOSES
EVEN-ODD NUCLEI
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MATERIALS
MONOCYTES
NUCLEI
PHYSIOLOGY
RADIATION DOSES
RADIATION EFFECTS
RADIOISOTOPES
SOMATIC CELLS
TISSUE CULTURES
TRACER TECHNIQUES