Inhibiting and stimulating effects of TGF-. beta. 1 on osteoclastic bone resorption in fetal mouse bone organ cultures

Journal Article · Wed May 01 04:00:00 EDT 1991 · Journal of Bone and Mineral Research; (United States)

OSTI ID:5394183

Dieudonne, S C; Foo, P; van Zoelen, E J; Burger, E H ^[1]

---

1. Department of Oral Cell Biology, Vrije Universiteit, Amsterdam (Netherlands)

The effects of TGF-{beta} 1 on osteoclastic resorption of fetal mouse calvaria and long bones at various stages of development was studied in organ culture. In resorbing calvariae and long bones with an established marrow cavity TGF-beta 1 (4-10 ng/ml) had a stimulating effect on 45Ca release that was partially inhibited by indomethacin. In primitive long bones, however, which were explanted before osteoclast invasion and excavation of a marrow cavity had started, TGF-beta 1 (1-4 ng/ml) inhibited 45Ca release by an indomethacin-insensitive mechanism. Histomorphometry of long bones after staining for tartrate-resistant acid phosphatase (TRAP) revealed that TGF-beta 1 treatment inhibited the migration of TRAP-positive cells from periosteum to developing marrow cavity and inhibited cell fusion. However, the formation of (mononuclear) TRAP-positive cells in the periosteum-perichondrium was strongly enhanced. These data suggest that TGF-beta 1 modulates various steps in the cascade of osteoclast development, recruitment, and activation in different ways, involving both prostaglandin-mediated and prostaglandin-independent pathways. Therefore the net effect of exogenous TGF-beta 1 on osteoclastic resorption in bone organ cultures depends on the relative prevalence of osteoclast progenitors, precursors, and mature osteoclasts in the tissue under study.

• OSTI ID: 5394183
• Journal Information: Journal of Bone and Mineral Research; (United States), Journal Name: Journal of Bone and Mineral Research; (United States) Vol. 6:5; ISSN JBMRE; ISSN 0884-0431
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
BODY
BONE CELLS
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FETUSES
GROWTH FACTORS
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
METALS
MICE
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROSTAGLANDINS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SECRETION
SKELETON
SOMATIC CELLS
SORPTION
TISSUE CULTURES
TRACER TECHNIQUES
VERTEBRATES