Effects of transforming growth factor beta 1 on the regulation of osteoclastic development and function

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Journal of Bone and Mineral Research; (USA)

OSTI ID:5545451

Hattersley, G; Chambers, T J ^[1]

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1. St. George's Hospital Medical School, London (United Kingdom)

Transforming growth factor (TGF) beta 1 is a multifunctional cytokine with powerful effects on osteoblastic cells. Its role in the regulation of osteoclast generation and function, however, is unclear. It has been reported both to stimulate and to inhibit resorption in organ culture and to inhibit multinuclear cell formation in bone marrow cultures. We tested the effects of TGF-beta 1 on bone resorption by osteoclasts isolated from neonatal rat long bones. We found potent stimulation of osteoclastic bone resorption, mediated by osteoblastic cells, with an EC50 of 10 pg/ml, considerably lower than that of well-documented osteotropic hormones. Stimulation was not mediated by Swiss mouse 3T3 cells, a nonosteoblastic cell line. TGF-beta 1 strongly inhibited the generation of calcitonin receptor (CTR)-positive cells in mouse bone marrow cultures, but as for isolated osteoclasts, bone resorption per CTR-positive cell was increased. The inhibition of CTR-positive cell formation was associated with suppression of maturation of other bone marrow derivatives and may be related more to the known ability of TGF-beta 1 to suppress the proliferation of primitive hematopoietic cells than to a specific role of TGF-beta 1 in osteoclast generation.

• OSTI ID: 5545451
• Journal Information: Journal of Bone and Mineral Research; (USA), Journal Name: Journal of Bone and Mineral Research; (USA) Vol. 6:2; ISSN JBMRE; ISSN 0884-0431
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL FUNCTIONS
BODY
BONE CELLS
BONE MARROW
CALCITONIN
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
HORMONES
IODINE ISOTOPES
ISOTOPES
MAMMALS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RATS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES