Onset of apoprotein E secretion during differentiation of mouse bone marrow-derived mononuclear phagocytes

Journal Article · Sat Oct 01 04:00:00 EDT 1983 · J. Cell Biol.; (United States)

DOI:https://doi.org/10.1083/jcb.97.4.1113· OSTI ID:6350073

Werb, Z; Chin, J R

A number of macrophage functions were sequentially expressed when the bone marrow precursors of mononuclear phagocytes differentiated in culture in the presence of a specific growth factor, colony-stimulating factor-1. The authors defined the expression of apoprotein E (ApoE), a major secreted protein of resident peritoneal macrophages, during maturation of adherent bone marrow-derived mononuclear phagocytes into macrophages. By 5 d the bone marrow macrophages were active secretory cells, but few cells contained intracellular immunoreactive ApoE, and little, if any, ApoE was secreted. ApoE secretion was initiated at 9 d, and this correlated with an increase in the percentage of macrophages containing intracellular ApoE. The onset of ApoE secretion was selective, and little change occurred in the other major secreted proteins detected by (/sup 35/S)methionine incorporation. In parallel, the high rate of plasminogen activator secretion, which peaked at 7 d, decreased markedly. ApoE secretion was not associated with altered expression of the macrophage surface antigen, la, or with secretion of fibronectin. Virtually all cells in independent colonies of bone marrow-derived macrophages eventually expressed ApoE. The proliferating monocyte/macrophage-like cell lines P388D1, J774.2, WHEI-3, RAW 264.1, and MGI.D/sup +/ secreted little or no ApoE. These data establish that ApoE secretion is developmentally regulated.

• Research Organization: Univ. of California, San Francisco
• DOE Contract Number: AC03-76SF01012
• OSTI ID: 6350073
• Journal Information: J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 97; ISSN JCLBA
• Country of Publication: United States
• Language: English

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550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BODY
BONE MARROW
CARBOXYLIC ACIDS
CELL DIFFERENTIATION
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
CYTOLOGY
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
LIPOTROPIC FACTORS
MACROPHAGES
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHAGOCYTES
PRECURSOR
PROTEINS
RADIOISOTOPES
SECRETION
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES