Differentiation kinetics of osteoclasts in the periosteum of embryonic bones in vivo and in vitro

Journal Article · Tue Apr 01 04:00:00 EST 1986 · Anat. Rec.; (United States)

DOI:https://doi.org/10.1002/ar.1092140413· OSTI ID:5687333

Scheven, B A; Kawilarang-De Haas, E W; Wassenaar, A M; Nijweide, P J

Osteoclast progenitors are seeded via the blood stream in the mesenchyme surrounding embryonic long bone models long before the appearance of multinucleated osteoclasts. The proliferation and differentiation of these progenitors in embryonic mouse metatarsal bones was studied with acid phosphatase (AcP) histochemistry and /sup 3/H-thymidine autoradiography. In vivo, tartrate-resistant, acid phosphatase-positive, mononuclear cells appear in the periosteum (AcPP-P cells) at the age of 17 days (after conception). On day 18, AcP-positive, multinucleated osteoclasts invade the bone rudiment and start resorbing the calcified cartilage matrix, resulting in the formation of the marrow cavity. The kinetics of osteoclast formation in vitro was studied in metatarsal bones of embryonic mice of different ages cultured in the continuous presence of /sup 3/H-thymidine. In young bones (15 days), mainly proliferating, /sup 3/H-thymidine-incorporating progenitors gave rise to AcPP-P cell and osteoclast formation. In older bones (16 and 17 days) osteoclasts were progressively more derived from postmitotic, unlabeled precursors. Irradiation of the metatarsal bones with a radiation dose of 5.0 Gy prior to culture resulted in a selective elimination of the proliferating progenitors, whereas the contribution of postmitotic precursors in AcPP-P cell and osteoclast formation remained unchanged. The results demonstrate that in the periosteum of embryonic metatarsal bones a shift occurs from a population composed of proliferating osteoclast progenitors (15 days) to a population composed of postmitotic precursors (17 days) before multinucleated osteoclasts are formed (18 days).

• Research Organization: Univ. of Leiden, The Netherlands
• OSTI ID: 5687333
• Journal Information: Anat. Rec.; (United States), Journal Name: Anat. Rec.; (United States) Vol. 4; ISSN ANREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID PHOSPHATASE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE TISSUES
CARTILAGE
CELL DIFFERENTIATION
CELL PROLIFERATION
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DOSES
EMBRYONIC CELLS
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
IN VITRO
IN VIVO
IRRADIATION
KINETICS
LABELLED COMPOUNDS
MAMMALS
MICE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHATASES
PRECURSOR
PYRIMIDINES
RADIATION DOSES
RADIATION EFFECTS
REACTION KINETICS
RIBOSIDES
RODENTS
SKELETON
SOMATIC CELLS
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES