Effects of ionizing irradiation on formation and resorbing activity of osteoclasts in vitro

Journal Article · Mon Jul 01 04:00:00 EDT 1985 · Lab. Invest.; (United States)

OSTI ID:5217727

Scheven, B A; Burger, E H; Kawilarang-de Haas, E W; Wassenaar, A M; Nijweide, P J

The effects of ionizing irradiation on the differentiation and activity of the osteoclast were investigated. Embryonic mouse metatarsal bones of different ages (14, 15, 16, 17 days) in which no osteoclasts had as yet been formed were irradiated with various x-ray doses and cultured until a marrow cavity became visible in the nonirradiated paired control bones. Bone growth and calcification were followed microscopically during culture. Irradiation caused a dose-dependent stunting of the longitudinal growth. Calcification was inhibited by high radiation doses (10 to 20 Gray (Gy), whereas a dose of 2.5 Gy stimulated the process in the early stages of long bone development. Histologic examination revealed complete inhibition of osteoclast formation in the 14- and 15-day-old bones after irradiation with 2.5 Gy or more. The number of osteoclasts in cultured older bones (16 days) was significantly reduced by irradiation, but osteoclast formation could not be completely prevented even by high dosages. Irradiation of explanted bone rudiments which were in a stage 1 day prior to the appearance of osteoclasts in vivo (17 days) did not significantly influence the formation of osteoclasts. Autoradiographic experiments using young bones showed that differentiation of osteoclast precursors into multinucleated osteoclasts is preceded by one or more divisions of the precursors in the periosteum. Furthermore, it was established from continuous /sup 3/H-thymidine-labeling experiments that in older bones (16 days) a part of the osteoclast nuclei originated from postmitotic osteoclast precursors. Irradiation mainly inhibited the appearance of labeled osteoclast nuclei in these bones.

• Research Organization: Leiden Univ., Netherlands
• OSTI ID: 5217727
• Journal Information: Lab. Invest.; (United States), Journal Name: Lab. Invest.; (United States) Vol. 1; ISSN LAINA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL DIVISION
DOSE-RESPONSE RELATIONSHIPS
DOSES
EMBRYONIC CELLS
EMBRYOS
IN VITRO
LABELLED COMPOUNDS
MAMMALS
MICE
ORGANS
RADIATION DOSES
RADIATION EFFECTS
RODENTS
SKELETON
SOMATIC CELLS
STEM CELLS
TRITIUM COMPOUNDS
VERTEBRATES