Further characterization of suppressor lymphocytes induced by fetal calf serum in murine lymphoid cell cultures: comparison with in vitro-generated cytotoxic lymphocytes. [X Radiation]

Journal Article · Thu Mar 15 04:00:00 EST 1979 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(79)90177-1· OSTI ID:6294829

Kedar, E; Schwartzach, M

Nonspecific suppressor cell (SPC) activity has been induced in vitro by preculturing splenocytes from normal mice in the presence of fetal calf serum (FCS) for 3 days or more. In adoptive transfer experiments in vivo, these precultured SPC were shown to reduce the humoral response of mice to SRBC and the cell-mediated cytotoxic (CMC) response to allogeneic tumor cells. In mixing experiments in vitro, using freshly explanted splenocytes, the precultured splenocytes abrogated the generation of specific cytotoxic lymphocytes (CL) in primary mixed lymphocyte-tumor cell cultures (MLTC). By contrast, secondary cytotoxic response was only marginally affected. Supernatants of precultured cells were also inhibitory, although to a less degree than whole cells. The induction of suppressive activity was abolished by addition of mitogenic amounts of concanavalin A to the preculturing medium. By the use of cell fractionation techniques it was found that both specific CL and nonspecific SPC lack an FC receptor, do not adhere to nylon wool, and cannot be separated from each other by density sedimentation on a discontinuous BSA gradient. However, precursors of SPC and CL differed in their susceptibility to cyclophosphamide, hydrocortisone, and irradiation. The data presented does not exclude the possibility that suppressive activity exerted by FCS-induced SPC is mediated through a cytotoxic effect.

• Research Organization: Hebrew Univ., Jerusalem, Israel
• OSTI ID: 6294829
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 43:2; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD SERUM
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DATA
DATA FORMS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
EXPERIMENTAL DATA
GLOBULINS
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
IMMUNE REACTIONS
INFORMATION
IONIZING RADIATIONS
ISOLATED VALUES
KETONES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MICE
NUMERICAL DATA
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
STEROID HORMONES
STEROIDS
TUMOR CELLS
VERTEBRATES
X RADIATION