Effects of. gamma. -irradiation on lymphocyte subpopulations participating in the development of the cytotoxic T lymphocyte response
Differences in radiosensitivity among lymphocyte subpopulations involved in generating allogeneic cytotoxic T lymphocyte (CTL) responses were studied. Gamma irradiated splenic lymphocytes from C57Bl/6 mice (B6, H-2/sup b/) were stimulated in vitro with DBA/2 (H-2/sup d/) spleen cells or the DBA/2 mastocytoma, P815. Limiting dilution analysis indicated that the cytotoxic T lymphocyte effector cells (CTLe) for either antigen arose from the same pool of cytotoxic T lymphocyte precursors (CTLp), and that the D/sub 37/ value of the B6 anti-H-2/sup d/ CTLp was approx. =165 R. In mixed lymphocyte cultures, however, differential effects of irradiation were seen between the 2 antigens in the ability of irradiated responder B6 spleen cells to generate CTLe. For induction of cytotoxic activity against DBA/2 splenocytes, the CTLp was the most radiosensitive component of the response, whereas the generation of a cytotoxic response against P815 was limited by the radiosensitivity of an Lyt-1 T helper cell (D/sub 37/approx. =85 R). The radiosensitive Lyt-1 cell could be replaced by mixed lymphocyte reaction supernatants or semipurified interleukin 2 (IL-2) from Con A-stimulated murine spleen cells. The requirement of B6 anti-H-2/sup d/ CTLp for a radiosensitive accessory cell in the CTL response against P815, in contrast with their activation by DBA/2 splenocytes, may center on the fact that the tumor cells do not possess Ia antigens.
- Research Organization:
- Memorial Sloan-Kettering Cancer Center, Rye, NY
- OSTI ID:
- 5571887
- Journal Information:
- J. Immunol.; (United States), Vol. 127:4
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANTIGEN-ANTIBODY REACTIONS
RADIOSENSITIVITY
LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
EXPERIMENTAL DATA
GAMMA RADIATION
IN VITRO
MICE
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DATA
ELECTROMAGNETIC RADIATION
INFORMATION
IONIZING RADIATIONS
LEUKOCYTES
MAMMALS
MATERIALS
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550300 - Cytology