Detection and characterization of cytotoxic T lymphocyte precursors in the murine intestinal intraepithelial leukocyte population
Highly purified preparations of intraepithelial leukocytes (IEL) were obtained from the small intestinal mucosa. Because approximately 80% of IEL expressed the Lyt-2 antigen usually associated with cytotoxic/suppressor T lymphocytes, the authors wished to determined if precursors for cytotoxic T cells were present in this population. In order to generate cytotoxic cells, IEL and spleen cells from CBA/J mice (H-2/sup k/) were co-cultured with irradiated allogeneic spleen cells (H-2/sup d/ or H-2/sup b/) in a one-way mixed leukocyte reaction (MLR). Four to six days later, the culture cells were assayed against /sup 51/Cr-labeled H-2/sup d/ or H-2/sup b/ tumor of Con A-stimulated lymphoblast target cells, and the specificity of alloantigen-stimulated IEL and spleen cells was compared. The cytotoxic cells derived from both tissues displayed antigen-specific lysis of the allogeneic targets. Treatment of effector cells, generated from intraepithelial or splenic precursors, with monoclonal antibodies against Thy-1.2, Lyt-1.1, or Lyt-2.1 antigens plus complement, decreased cytotoxicity 85 to 100%, event though only 20 to 50% of the cells were lysed. The alloantigen specificity and surface antigen phenotype of the cultured IEL cells were identical to those of spleen cells and allowed them to conclude that IEL contained a cytotoxic T lymphocyte precursor (CTLp).
- Research Organization:
- McMaster Univ. Health Sciences Centre, Hamilton, Ontario
- OSTI ID:
- 7085465
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:6; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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