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In vitro generation of nonspecific suppressor cells and their characterization. [X radiation]

Journal Article · · Transplantation; (United States)
OSTI ID:6252610
In vitro preculture of C3H/He splenocytes for 48 to 96 hr induces aspecific suppressor cells evaluated by their ability to reduce /sup 3/H-TdR uptake by fresh syngeneic splenocytes stimulated by different amounts of Concanavalin A (Con A) in vitro. This suppressive activity is obtained in medium containing 10% heat-inactivated fetal calf serum (FCS) or similar amounts of heat-inactivated bovine serum, syngeneic, or allogeneic murine sera but not by unheated FCS. Suppressive activity is resistant to mitomycin C and radiation up to 5,000 R. Exposure of precultured cells to carbonyl iron or plastic adherence, but not to anti-Thy 1.2 serum plus complement, results in removal of the suppressive activity.
Research Organization:
Istituto di Ricerche Farmacologiche, Milan, Italy
OSTI ID:
6252610
Journal Information:
Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 27:5; ISSN TRPLA
Country of Publication:
United States
Language:
English

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