Comparison of the suppressor cells found in the spleens of 89Sr-treated mice and in normal murine bone marrow

Journal Article · Sun Mar 01 04:00:00 EST 1981 · J. Immunol.; (United States)

OSTI ID:6025635

Levy, E M; Corvese, J S; Bennett, M

Normal murine bone marrow cells and spleen cells of mice treated with 89Sr both have suppressive activity. These nonspecific suppressor cells inhibit the ability of normal spleen cells to undergo antibody responses in vitro. After being precultured for 24 h, these cells will also suppress antibody responses in vivo and the responses of normal spleen cells to T and B cell mitogens in vitro. These cells have previously been shown not to be mature T or B lymphocytes or macrophages. Velocity sedimentation and cell-size analysis indicated that both suppressor cells are large (approximately 206 mu/sup 3/). Mitomycin C treatment eliminated the ability of both suppressor cells to inhibit an in vitro antibody response. In contrast, this treatment did not reduce the ability of the cells to suppress a mitogenic response. Irradiation (1000 R) was also ineffective in eliminating the ability of either cell to suppress a mitogenic response. We conclude that the 2 suppressor cells are closely related if not identical, and we speculate that these cells may function in vivo to suppress immune reactivity in areas of intense hematopoiesis.

• OSTI ID: 6025635
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 126:3; ISSN JOIMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD FORMATION
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOSES
EVEN-ODD NUCLEI
IN VIVO
INTERMEDIATE MASS NUCLEI
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MAMMALS
MICE
MITOGENS
NUCLEI
PHYSIOLOGY
RADIATION DOSES
RADIATION EFFECTS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
STRONTIUM 89
STRONTIUM ISOTOPES
VERTEBRATES