Regulation of delayed-type hypersensitivity: VI. Antigen-specific suppressor T cells and suppressor factor for delayed-type hypersensitivity to histocompatibility antigens
Mice develop highly significant levels of delayed-type hypersensitivity (DTH) to major and minor histocompatibility antigens when injected s.c. with lymphoid cells from X-irradiated allogeneic donors. However, when mice are inoculated i.v. with a high dose of X-irradiated allogeneic lymphoid cells, they not only fail to develop DTH to the allogeneic cells, but their ability to respond to an immunogenic challenge of the alloantigens is also significantly depressed. This suppression is adoptively transferable by antigen-specific suppressor T cells and not by immune serum. Cell surface phenotypic analysis shows that the primary suppressor cells for alloantigens are Thy-1/sup +/, Lyt-1/sup +/2/sup -/, and Ia/sup -/, whereas the secondary suppressor cells appearing after boosting injection are Thy-/sup +/, Lyt-1/sup +/2/sup +/, and Ia/sup -/. These suppressor T (Ts) cells localize in the lymphoid organs shortly after their induction and are largely absent from the spleen or lymph node 1 month later.However, ''suppressor memory'' can be recalled by an immunogenic dose of alloantigens which would normally induce DTH effector cells rather than suppressor cells in naive mice. When the suppressor cells were cultured in vitro for 48 hr, the supernatant contained suppressive activity. It appears likely that the manifestation of the suppressor cells is via soluble, antigen-specific suppressor factor(s), the production of which is dependent on viable T cells.
- Research Organization:
- Wellcome Research Laboratories, Kent, England
- OSTI ID:
- 6715928
- Journal Information:
- Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 33:1; ISSN TRPLA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Suppression of antigraft immunity by preimmunization. II. Characterization of the suppressor cells
Regulatory action of immune B cells on delayed-type hypersensitivity in mice
Related Subjects
560152 -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IMMUNITY
IMMUNOLOGY
IMMUNOSUPPRESSION
INJECTION
INTAKE
INTRAVENOUS INJECTION
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
SUBCUTANEOUS INJECTION
VERTEBRATES
X RADIATION