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Production of antigen-specific suppressive T cell factor by radiation leukemia virus-transformed suppressor T cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
 [1]; ;
  1. Univ. of Milan, Italy
+ Show Author Affiliations

Hen egg-white lysozyme-specific suppressor T cells induced in C57BL/6 mice have been selected by sequential passage over plates coated with goat anti-mouse Ig and HEL. These suppressor T cells, 80% I-J/sup +/, were infected in vitro with radiation leukemia virus and injected intravenously into sublethally irradiated syngeneic recipients. After 4 to 6 months, 6 out of 20 injected mice developed thymic lymphomas, which were maintained by transplantation into histocompatible hosts and subsequently established as permanent cell lines. Cells of these six thymomas were screened for the presence of Thy 1.2, Lyt 1, Lyt 2, I-J/sup b/, and Ig cell surface antigens by direct or indirect immunofluorescence. One tumor was found to express the expected phenotype of suppressor T cells. High-speed supernatants of extracts obtained from L/sub 4/ cells were able to induce HEL-specific suppression in a T cell proliferative assay, demonstrating the presence of an antigen-specific suppressive T cell factor.

OSTI ID:
6195825
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:6; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560152 -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CELL CULTURES
CELL PROLIFERATION
CHEMISTRY
DISEASES
ELECTROMAGNETIC RADIATION
ENZYMES
GLYCOSYL HYDROLASES
GROWTH
HEMIC DISEASES
HYDROLASES
IMMUNE REACTIONS
IN VITRO
INJECTION
INTAKE
IONIZING RADIATIONS
LEUKEMIA
LYMPHOMAS
LYSOZYME
MAMMALS
MICE
MICROORGANISMS
MORPHOLOGY
NEOPLASMS
O-GLYCOSYL HYDROLASES
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
SYNTHESIS
THYMUS CELLS
TIME DEPENDENCE
VERTEBRATES
VIRUSES
X RADIATION