Immortalization of antigen specific, helper T cell lines by transformation with the radiation leukemia virus (RadLV)
Antigen-specific immune T lymphocytes of male C57BL/6 mice were enriched in vitro on monolayers of antigen-pulsed syngeneic macrophages. The cells were treated in vitro with RadLV and inoculated intrathymically into irradiated female C56BL/6 animals. Thymomas arising in the inoculated recipients were characterized as donor- (male) type according to their karyotype. In vivo lymphomas, two of which (designated ROT/6.1 and ROT/6.2) were capable of providing antigen- (carrier) specific help in normal or preimmunized mice. None of the lymphomas could induce antigen-specific DTH reaction. Five months after their establishment, ROT/6.2 alone retained its carrier specificity. ROT/6.2 consisted mainly of Lyt-1/sup +/ cells, whereas the ROT/6.1 population was more heterogeneous and contained Lyt-1/sup +/, Lyt-2/sup +/, and Lyt-3/sup +/ cells. The carrier specificity of the latter may have been lost due to selection against the specific helper cells during prolonged passages.
- Research Organization:
- The Hebrew Univ.-Hadassah Medical School, Jerusalem
- OSTI ID:
- 5636197
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 128:2; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
HEMIC DISEASES
IMMUNE REACTIONS
IN VITRO
IONIZING RADIATIONS
IRRADIATION
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
MICROORGANISMS
NEOPLASMS
ONCOGENIC VIRUSES
PARASITES
PHAGOCYTES
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SPECIFICITY
VERTEBRATES
VIRUSES
WHOLE-BODY IRRADIATION
X RADIATION