Quantitation, in vitro propagation, and characterization of preleukemic cells induced by radiation leukemia virus
- Hebrew Univ.-Hadassah Medical School, Jerusalem (Israel)
Intrathymic (i.t.) inoculation of radiation leukemia virus into C57BL/6 mice induces a population of preleukemic (PL) cells that can progress into mature thymic lymphomas upon transfer into syngeneic recipients. A minimum of 10(3) PL thymic cells are required to induce lymphomas in the recipient. Most of the individual lymphomas developed in mice which were inoculated with cells of a single PL thymus, derived from different T-cell precursors. PL thymic cells could be grown in vitro on a feeder layer consisting of splenic stromal cells. Growth medium was supplemented with supernatant harvested from an established radiation leukemia virus-induced lymphoma cell line (SR4). The in vitro-grown PL cells were characterized as Thy-1+, CD4+, CD8- T-cells, most of which expressed radiation leukemia virus antigens. Cultured PL cells were found to be nontumorigenic, based on their inability to form s.c. tumors. However, these cells could develop into thymic lymphomas if inoculated i.t. into syngeneic recipients. A culture of PL cells, maintained for 2 mo, showed clonal T-cell receptor arrangement. Lymphomas which developed in several recipient mice upon injection with these PL cells were found to possess the same T-cell receptor arrangement. These results indicate that PL cells can be adapted for in vitro growth while maintaining their preleukemic character.
- OSTI ID:
- 5800448
- Journal Information:
- Cancer Research; (USA), Journal Name: Cancer Research; (USA) Vol. 51:8; ISSN 0008-5472; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BODY
DISEASES
DNA
EXPERIMENTAL NEOPLASMS
IMMUNE SYSTEM DISEASES
IN VITRO
IRRADIATION
LEUKEMIA VIRUSES
LYMPHATIC SYSTEM
LYMPHOMAS
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
RADIOINDUCTION
RODENTS
SOMATIC CELLS
THYMUS
THYMUS CELLS
TUMOR CELLS
VERTEBRATES
VIRUSES