Differential antibody production by adherent and nonadherent spleen cells transferred to irradiated and cyclophosphamide-treated recipient mice. [X radiation]

Journal Article · Tue Apr 01 04:00:00 EDT 1975 · RES, J. Reticuloendothel. Soc.; (United States)

OSTI ID:7120475

Albright, J F ^[1]; Deitchman, J W; Hassell, S A; Ozato, K

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1. Indiana State Univ., Terre Haute

Mouse spleen cells were separated into adherent (Ad) and nonadherent (Nad) populations by incubation in plastic petri dishes. Adherent, Nad and unfractionated cell preparations (UCP) were transferred into syngeneic recipient mice that had been either irradiated or cyclophosphamide (CY) treated and the adoptive humoral Ab responses were studied by assessment of hemolytic Ab-forming cells (PFC) or humoral serum Ab production. Adherent cells failed to produce PFC in irradiated recipients, but functioned vigorously in CY-treated recipients. Nonadherent cells generated PFC in either type of host, as did UCP. Studies of comparative responses in CY-treated recipients revealed that: (a) Ad-cells generated /sup 2///sub 3/ the number of PFC given by equivalent numbers of transferred Nad cells and UCP; (b) per equivalent numbers of transferred cells the Ad fraction generated 5 times more and 16 times more Ab than did the Nad cells and UCP, respectively. Spleen cells taken from mice 6 hr after CY treatment failed to respond to the mitogens phytohemagglutinin and bacterial lipopolysaccharide, showing that all cells were temporarily incapable of proliferation. Transfer of spleen cells from donor mice 16 hr after CY treatment, into thymectomized, irradiated, bone marrow-reconstituted recipients revealed substantial T-helper cell activity. We conclude that: (a) Ad preparations lacked T cells that were supplied by CY-treated recipients although T cell proliferation was temporarily inhibited in the latter; (b) B cells present in the Ad fraction were removed from some type of inhibitor of Ab synthesis and/or secretion, the production of which may be associated with T cells present in Nad preparations and UCP; (c) T-helper cells were only transiently affected by CY.

• OSTI ID: 7120475
• Journal Information: RES, J. Reticuloendothel. Soc.; (United States), Journal Name: RES, J. Reticuloendothel. Soc.; (United States) Vol. 17:4; ISSN RESJA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ALKYLATING AGENTS
ANIMAL CELLS
ANTIBODIES
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
CARBOHYDRATES
CELL PROLIFERATION
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
HEMAGGLUTININS
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IONIZING RADIATIONS
LIPIDS
LIPOPOLYSACCHARIDES
MEDICINE
MUCOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
SOMATIC CELLS
SPLEEN CELLS
SURGERY
THYMECTOMY
TISSUES
X RADIATION