Comparative study regarding the association of alpha-2U globulin with the nephrotoxic mechanism of certain petroleum-based Air Force fuels. Final report, 1 September 1986-31 August 1987

Technical Report · Tue Oct 27 04:00:00 EST 1987

OSTI ID:7252559

Eurell, T E

Alpha-2U globulin is a low-molecular-weight urinary protein which may be associated with a hydrocarbon-induced proximal tubular cell degeneration in the male rat kidney. A new method was developed to obtain monospecific immunologic reagents for alpha-2U globulin using diafiltration, anion exchange and hydroxylapatite chromatography. Isoelectric focusing techniques were developed to isolate the major isoelectric variants of the alpha-2U globulin molecule and to assess changes in alpha-2U globulin after experimental exposure to hydrocarbon compounds. Alpha-2U globulin was isolated from the urine of albino and pigmented male rats to study strain susceptibility to the nephrotoxic process. An alpha-2u globulin isoelectric variant profile distinguishing albino from non-albino male rats was not apparent, however, strain differences were revealed. Fischer 344 male rats appear to have higher levels of the isoelectric variants than the other strains studied. These findings suggest that if a strain susceptibility to the hydrocarbon-induced nephrotoxic lesion exists, it may be associated with the alpha-2U globulin isoelectric variant profile.

• Research Organization: Illinois Univ., Urbana (USA). Dept. of Veterinary Biosciences
• OSTI ID: 7252559
• Report Number(s): AD-A-190532/2/XAB
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020900 -- Petroleum-- Environmental Aspects
550900 -- Pathology
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
AVIATION FUELS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
DOCUMENT TYPES
FUELS
GLOBULINS
GLOBULINS-ALPHA
HYDROCARBONS
IMMUNOLOGY
KIDNEYS
MALES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
PETROLEUM PRODUCTS
PROGRESS REPORT
PROTEINS
RATS
RODENTS
TOXICITY
URINE
VERTEBRATES
WASTES