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Radiosensitivity of hematopoietic stem cells in diffusion chamber cultures of the murine yolk sac and adult medullary tissue

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3574536· OSTI ID:7245519
;
The radiosensitivity of hematopoietic progenitor cells derived from the 10/sup 1///sub 2/ day murine yolk sac and adult murine bone marrow have been compared. A yolk sac cell suspension was exposed to varying amounts of gamma radiation (0 to 950 R) and the index used for radiosensitivity was the proliferation and differentiation of these cells cultured for 10 days in the millipore diffusion chamber. The data provided evidence that the hematopoietically active embryonic yolk sac is less radiosensitive than the adult mouse tibial marrow cells. A 10/sup 1///sub 2/ day yolk sac cell suspension (5.182 +- 0.601 x 10/sup 6/ cells per yolk sac) contained at least 2.8 +- 1.1 pluripotential stem cells (colony-forming units, CFU) and 242 +- 31 committed stem cells (colony-forming cells, CFC). The results suggest the existence of a hematopoietic stem cell compartment in the yolk sac which is markedly different from that found in adult medullary tissue. Furthermore, there is a difference between the expressions of the differentiation potential of precursor cells of extraembryonic and of adult origin when exposed to similar microenvironments.
Research Organization:
Saint Elizabeth's Hospital of Boston
OSTI ID:
7245519
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 68:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BODY
BONE MARROW
CELL CULTURES
COMPARATIVE EVALUATIONS
EGGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HEMATOPOIETIC SYSTEM
IONIZING RADIATIONS
IRRADIATION
ORGANS
RADIATIONS
RADIOSENSITIVITY
SOMATIC CELLS
STEM CELLS
TISSUES