Observations on the contributions of environmental restraints and innate stem cell ability to hematopoietic regeneration
Journal Article
·
· Int. J. Cell Cloning; (United States)
A competitive repopulation assay utilizing chromosome markers was used to assay the reconstituting potential of hematopoietic populations. The test populations consisted of tibial murine marrow locally irradiated with doses ranging from 1.5 Gy to 8.5 Gy and of marrow generated from either murine splenic or marrow stem cells. The purpose of this assay was to assess the innate proliferative potential and microenvironmental influences on the ability to repopulate. Regardless of origin, spleen repopulating ability consistently agreed with spleen colony-forming unit (CFU-s) content. Doses of radiation from 5 Gy to 8.5 Gy diminished, by a factor of 2, the ability to repopulate marrow despite maintenance of CFU-s levels. Marrow generated from splenic stem cells had one-fifth the repopulating ability of marrow derived from marrow stem cells, even though CFU-s levels were equivalent. The results imply that the splenic environment can only maintain stem cells at the level of the CFU-s, even if the stem cells were originally of higher quality, and that their original potential cannot be regained in a marrow environment. Nevertheless, the marrow can maintain more primitive stem cells, but this reserve is drained to support CFU-s levels.
- Research Organization:
- Hebrew Univ.-Hadassah Hospital Medical School, Jerusalem (Israel)
- OSTI ID:
- 6971604
- Journal Information:
- Int. J. Cell Cloning; (United States), Journal Name: Int. J. Cell Cloning; (United States) Vol. 6:2; ISSN IJCCE
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOASSAY
BIOLOGICAL MARKERS
BIOLOGICAL RECOVERY
BODY
BONE MARROW
CELL PROLIFERATION
COLONY FORMATION
DOSES
HEMATOPOIETIC SYSTEM
MAMMALS
MICE
ORGANS
RADIATION DOSES
RADIOSENSITIVITY
RECOVERY
RODENTS
SOMATIC CELLS
SPLEEN CELLS
STEM CELLS
TISSUES
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOASSAY
BIOLOGICAL MARKERS
BIOLOGICAL RECOVERY
BODY
BONE MARROW
CELL PROLIFERATION
COLONY FORMATION
DOSES
HEMATOPOIETIC SYSTEM
MAMMALS
MICE
ORGANS
RADIATION DOSES
RADIOSENSITIVITY
RECOVERY
RODENTS
SOMATIC CELLS
SPLEEN CELLS
STEM CELLS
TISSUES
VERTEBRATES