Heterogeneity within the hematopoietic stem cell compartment: evidence for a marrow-seeding stem cell distinct from CFU-s

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Int. J. Cell Cloning; (United States)

DOI:https://doi.org/10.1002/stem.5530030107· OSTI ID:5608127

Duke-Cohan, J S; Davies, A J; Wallis, V J

Using a chromosome marker within a syngeneic system, we investigated the seeding characteristics of murine hematopoietic stem cells after transplantation to irradiated hosts. The chromosome-marked test cells were allowed to compete with normal marrow cells in repopulating the spleen and marrow of irradiated mice. Although the seeding behavior of normal marrow could be predicted from the number of colony-forming units-spleen (CFU-s) transplanted, the marrow seeding of melphalan-treated marrow was 7-fold greater than expected. Repopulation of marrow by spleen cells was less effective than expected from the CFU-s content, while the reverse was true after repopulation by fetal liver cells. These differences were emphasized after treatment of cell donors with melphalan. The results were due primarily to differences in the lodging properties of the transplanted cells, those seeding in the marrow were less sensitive to melphalan than CFU-s. In some instances marrow-repopulating ability could be separated from peak CFU-s activity on a density gradient, suggesting a marrow-repopulating cell exists that is distinct from CFU-s.

• Research Organization: Chester Beatty Research Institute, London, England
• OSTI ID: 5608127
• Journal Information: Int. J. Cell Cloning; (United States), Journal Name: Int. J. Cell Cloning; (United States) Vol. 3:1; ISSN IJCCE
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW CELLS
CHIMERAS
CONNECTIVE TISSUE CELLS
EMBRYONIC CELLS
HEMATOPOIETIC SYSTEM
MAMMALS
MICE
MOSAICISM
RADIATION CHIMERAS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
STEM CELLS
TRANSPLANTS
VERTEBRATES