Radiosensitivity of murine hemopoietic colony-forming units assayed in situ in the rib and in other marrow sites

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576692· OSTI ID:5528288

Hendry, J H; Schofield, R; Bwire, N E

The radiosensitivity of murine hemopoietic colony-forming cells, which produce colonies in situ and which were counted at Day 8 after irradiation in sections of the femur, humerus, sternum, and spleen, is characterized by a D0 value of 91 +/- 9 cGy. The radiosensitivity of such cells in the rib was assessed using a new technique measuring regeneration or ablation of marrow in transverse sections of ribs observed at Day 8 after irradiation. The mean D0 value over a range determined using several different criteria was 108 cGy. These results provide evidence for the common assumptions that radiosensitivity measured using conventional transplantation assays reflects radiosensitivity in situ, and that the radiosensitivity of stem cells in different medullary marrow sites is similar. The techniques could be used with other species where assays for stem cells are not available.

• Research Organization: Christie Hospital and Holt Radium Institute, Manchester, England
• OSTI ID: 5528288
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
BONE MARROW
COLONY FORMATION
DOSE-RESPONSE RELATIONSHIPS
FEMUR
HEMATOPOIETIC SYSTEM
MAMMALS
MICE
ORGANS
RADIOSENSITIVITY
RODENTS
SKELETON
SOMATIC CELLS
SPLEEN
STEM CELLS
TISSUES
VERTEBRATES