In vitro studies on the radiosensitivity of multipotent hemopoietic progenitors in canine bone marrow
Journal Article
·
· Experimental Hematology (Lawrence, Kansas); (United States)
OSTI ID:5187236
- Institute for Occupational and Social Medicine, University of Ulm (West Germany)
The in vitro radiation response to 280-kV x-rays (does rate 72 cGy/min) of multipotent hemopoietic progenitor cells, mixed colony-forming units (CFU-mix), from canine bone marrow was assayed and compared to the radiation response characteristics of early erythroid progenitors, erythroid burst-forming units (BFU-E). To improve the colony-forming efficiency, the effect of various bone marrow cell separation techniques on colony formation of both progenitors was examined. The separation of bone marrow aspirates by discontinuous buoyant gradient centrifugation using the lymphocyte separation medium Lymphoprep with a density of 1.070 g/ml allowed the establishment of reproducible survival curves. The survival curves for both progenitors were strictly exponential, and CFU-mix were found to be more radiosensitive (D0 = 12 {plus minus} 2 cGy) than BFU-E (D0 = 16 {plus minus} 2 cGy).
- OSTI ID:
- 5187236
- Journal Information:
- Experimental Hematology (Lawrence, Kansas); (United States), Journal Name: Experimental Hematology (Lawrence, Kansas); (United States) Vol. 19:8; ISSN EXHMA; ISSN 0301-472X
- Country of Publication:
- United States
- Language:
- English
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·
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CENTRIFUGATION
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DOGS
ELECTROMAGNETIC RADIATION
IN VITRO
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
RADIATIONS
RADIOSENSITIVITY
SEPARATION PROCESSES
SOMATIC CELLS
STEM CELLS
ULTRACENTRIFUGATION
VERTEBRATES
X RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CENTRIFUGATION
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DOGS
ELECTROMAGNETIC RADIATION
IN VITRO
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
RADIATIONS
RADIOSENSITIVITY
SEPARATION PROCESSES
SOMATIC CELLS
STEM CELLS
ULTRACENTRIFUGATION
VERTEBRATES
X RADIATION