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Title: Radiation-induced DNA damage in canine hemopoietic cells and stromal cells as measured by the comet assay

Abstract

Stromal cell progenitors (fibroblastoid colony-forming unit; CFU-Fs) are representative of the progenitor cell population of the hemopoietic microenvironment in bone marrow (BM). Previous studies of the radiation dose-effect relationships for colony formation have shown that canine CFU-Fs are relatively radioresistant as characterized by a D{sub 0} value of about 2.4 Gy. In contrast, hemopoietic progenitors are particularly radiosensitive (D{sub 0} values = 0.12-0.60 Gy). In the present study, the alkaline single-cell gel electrophoresis technique for the in situ quantitation of DNA strand breaks and alkalilabile site was employed. Canine buffy coat cells from BM aspirates and cells harvested from CFU-F colonies or from mixed populations of adherent BM stromal cell (SC) layers were exposed to increasing doses of X-rays, embedded in agarose gel on slides, lysed with detergents, and placed in an electric field. DNA migrating from single cells in the gel was made visible as {open_quotes}comets{close_quotes} by ethidium bromide staining. Immediate DNA damage was much less in cultured stromal cells than in hemopoietic cells in BM aspirates. These results suggest that the observed differences in clonogenic survival could be partly due to differences in the type of the initial DNA damage between stromal cells and hemopoietic cells. 37 refs.,more » 2 figs., 1 tab.« less

Authors:
; ; ;  [1]
  1. Univ. of Ulm (Germany)
Publication Date:
OSTI Identifier:
426096
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental and Molecular Mutagenesis; Journal Volume: 27; Journal Issue: 1; Other Information: PBD: 1996
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; BONE MARROW; GENETIC RADIATION EFFECTS; DNA; STRAND BREAKS

Citation Formats

Kreja, L., Selig, C., Plappert, U., and Nothdurft, W. Radiation-induced DNA damage in canine hemopoietic cells and stromal cells as measured by the comet assay. United States: N. p., 1996. Web. doi:10.1002/(SICI)1098-2280(1996)27:1<39::AID-EM6>3.3.CO;2-1.
Kreja, L., Selig, C., Plappert, U., & Nothdurft, W. Radiation-induced DNA damage in canine hemopoietic cells and stromal cells as measured by the comet assay. United States. doi:10.1002/(SICI)1098-2280(1996)27:1<39::AID-EM6>3.3.CO;2-1.
Kreja, L., Selig, C., Plappert, U., and Nothdurft, W. Tue . "Radiation-induced DNA damage in canine hemopoietic cells and stromal cells as measured by the comet assay". United States. doi:10.1002/(SICI)1098-2280(1996)27:1<39::AID-EM6>3.3.CO;2-1.
@article{osti_426096,
title = {Radiation-induced DNA damage in canine hemopoietic cells and stromal cells as measured by the comet assay},
author = {Kreja, L. and Selig, C. and Plappert, U. and Nothdurft, W.},
abstractNote = {Stromal cell progenitors (fibroblastoid colony-forming unit; CFU-Fs) are representative of the progenitor cell population of the hemopoietic microenvironment in bone marrow (BM). Previous studies of the radiation dose-effect relationships for colony formation have shown that canine CFU-Fs are relatively radioresistant as characterized by a D{sub 0} value of about 2.4 Gy. In contrast, hemopoietic progenitors are particularly radiosensitive (D{sub 0} values = 0.12-0.60 Gy). In the present study, the alkaline single-cell gel electrophoresis technique for the in situ quantitation of DNA strand breaks and alkalilabile site was employed. Canine buffy coat cells from BM aspirates and cells harvested from CFU-F colonies or from mixed populations of adherent BM stromal cell (SC) layers were exposed to increasing doses of X-rays, embedded in agarose gel on slides, lysed with detergents, and placed in an electric field. DNA migrating from single cells in the gel was made visible as {open_quotes}comets{close_quotes} by ethidium bromide staining. Immediate DNA damage was much less in cultured stromal cells than in hemopoietic cells in BM aspirates. These results suggest that the observed differences in clonogenic survival could be partly due to differences in the type of the initial DNA damage between stromal cells and hemopoietic cells. 37 refs., 2 figs., 1 tab.},
doi = {10.1002/(SICI)1098-2280(1996)27:1<39::AID-EM6>3.3.CO;2-1},
journal = {Environmental and Molecular Mutagenesis},
number = 1,
volume = 27,
place = {United States},
year = {Tue Dec 31 00:00:00 EST 1996},
month = {Tue Dec 31 00:00:00 EST 1996}
}