Radiation-induced DNA damage in canine hemopoietic cells and stromal cells as measured by the comet assay

Journal Article · Tue Dec 31 04:00:00 EST 1996 · Environmental and Molecular Mutagenesis

DOI:https://doi.org/10.1002/(SICI)1098-2280(1996)27:1<39::AID-EM6>3.3.CO;2-1· OSTI ID:426096

Kreja, L; Selig, C; Plappert, U; Nothdurft, W ^[1]

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1. Univ. of Ulm (Germany)

Stromal cell progenitors (fibroblastoid colony-forming unit; CFU-Fs) are representative of the progenitor cell population of the hemopoietic microenvironment in bone marrow (BM). Previous studies of the radiation dose-effect relationships for colony formation have shown that canine CFU-Fs are relatively radioresistant as characterized by a D{sub 0} value of about 2.4 Gy. In contrast, hemopoietic progenitors are particularly radiosensitive (D{sub 0} values = 0.12-0.60 Gy). In the present study, the alkaline single-cell gel electrophoresis technique for the in situ quantitation of DNA strand breaks and alkalilabile site was employed. Canine buffy coat cells from BM aspirates and cells harvested from CFU-F colonies or from mixed populations of adherent BM stromal cell (SC) layers were exposed to increasing doses of X-rays, embedded in agarose gel on slides, lysed with detergents, and placed in an electric field. DNA migrating from single cells in the gel was made visible as {open_quotes}comets{close_quotes} by ethidium bromide staining. Immediate DNA damage was much less in cultured stromal cells than in hemopoietic cells in BM aspirates. These results suggest that the observed differences in clonogenic survival could be partly due to differences in the type of the initial DNA damage between stromal cells and hemopoietic cells. 37 refs., 2 figs., 1 tab.

• OSTI ID: 426096
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: 1 Vol. 27; ISSN EMMUEG; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BONE MARROW
DNA
GENETIC RADIATION EFFECTS
STRAND BREAKS