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Reaction of benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide with human hemoglobin and chromatographic resolution of the covalent adducts

Technical Report ·
DOI:https://doi.org/10.2172/7244688· OSTI ID:7244688
;

The formation of hemoglobin-carcinogen adducts has been proposed as a measure of human exposure to carcinogens. Hemoglobin-carcinogen adducts have been detected in carcinogen-treated animals and in human populations. Although polycyclic aromatic hydrocarbons (PAH) are ubiquitous in the human environment and DNA-PAH adducts have been detected in human tissues, the occurrent of hemoglobin-PAH adducts in humans has not been described. In this study we examined the effects of reaction conditions on the extent of in vitro reaction of human hemoglobin and (+)(anti)-({sup 3}H)benzo(a)pyrene-7,8-diol-9,10-epoxide (BPDE), a metabolite largely responsible for the carcinogenic effect of benzo(a)pyrene. The chromatographic properties of the resulting hemoglobin-BPDE adducts were examined by conventional DEAE-cellulose ion-exchange liquid chromatography and by reversed-phase high-performance liquid chromatography. Several adducts were chromatographically resolved from hemoglobin and from the individual globins.

Research Organization:
Argonne National Lab., IL (USA)
Sponsoring Organization:
DOE/ER
DOE Contract Number:
W-31109-ENG-38
OSTI ID:
7244688
Report Number(s):
ANL/PPRNT-90-202; ON: DE90008705
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADDUCTS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CONDENSED AROMATICS
DISEASES
ENVIRONMENTAL EXPOSURE
EPOXIDES
GLOBINS
HAZARDS
HEALTH HAZARDS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HUMAN POPULATIONS
HYDROCARBONS
IN VITRO
ION EXCHANGE CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
POPULATIONS
PORPHYRINS
PROTEINS
RESOLUTION
SEPARATION PROCESSES
TISSUES