Reaction of benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide with human hemoglobin and chromatographic resolution of the covalent adducts

Technical Report · Mon Jan 01 00:00:00 EST 1990

DOI:https://doi.org/10.2172/7244688· OSTI ID:7244688

Haugen, D. A.; Myers, S. R.

The formation of hemoglobin-carcinogen adducts has been proposed as a measure of human exposure to carcinogens. Hemoglobin-carcinogen adducts have been detected in carcinogen-treated animals and in human populations. Although polycyclic aromatic hydrocarbons (PAH) are ubiquitous in the human environment and DNA-PAH adducts have been detected in human tissues, the occurrent of hemoglobin-PAH adducts in humans has not been described. In this study we examined the effects of reaction conditions on the extent of in vitro reaction of human hemoglobin and (+)(anti)-({sup 3}H)benzo(a)pyrene-7,8-diol-9,10-epoxide (BPDE), a metabolite largely responsible for the carcinogenic effect of benzo(a)pyrene. The chromatographic properties of the resulting hemoglobin-BPDE adducts were examined by conventional DEAE-cellulose ion-exchange liquid chromatography and by reversed-phase high-performance liquid chromatography. Several adducts were chromatographically resolved from hemoglobin and from the individual globins.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 7244688
• Report Number(s): ANL/PPRNT-90-202; ON: DE90008705
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADDUCTS
ION EXCHANGE CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
BENZOPYRENE
BIOLOGICAL EFFECTS
EPOXIDES
CHEMICAL REACTIONS
HEMOGLOBIN
HUMAN POPULATIONS
HEALTH HAZARDS
ANIMALS
ENVIRONMENTAL EXPOSURE
IN VITRO
NEOPLASMS
POLYCYCLIC AROMATIC HYDROCARBONS
RESOLUTION
TISSUES
AROMATICS
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CONDENSED AROMATICS
DISEASES
GLOBINS
HAZARDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PIGMENTS
POPULATIONS
PORPHYRINS
PROTEINS
SEPARATION PROCESSES
550200* - Biochemistry