Mechanism of phage phi X174 DNA inactivation by benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide

Journal Article · Mon Aug 01 04:00:00 EDT 1977 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.74.8.3335· OSTI ID:5794676

Hsu, W T; Lin, E J.S.; Harvey, R G; Weiss, S B

A previous report from this laboratory has shown that certain derivatives of polycyclic aromatic hydrocarbons bind to phi X174 DNA and render it noninfectious. The present work describes the relationship between the extent of phi X174 DNA binding by (+-)-anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide and the effect on infectivity. The results suggest that one molecule of bound diolepoxide is sufficient to inhibit the replication of a single molecule of phi X174 DNA. DNA synthesis studies, in vitro, indicate that when phi X DNA bound by benzo(a)pyrene groups serves as template the rate of DNA polymerization is reduced and less product is formed. In addition, the propagation of synthetic DNA strands is blocked so that incomplete complementary chains are assembled. The relationship of these findings to the mutagenic and carcinogenic process associated with the action of benzo(a)pyrene-diol-epoxide is discussed.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 5794676
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 74:8; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARCINOGENESIS
CONDENSED AROMATICS
DNA
DNA REPLICATION
EPOXIDES
HYDROCARBONS
INACTIVATION
INFECTIVITY
MICROORGANISMS
MUTAGENESIS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PARASITES
PATHOGENESIS
POLYCYCLIC AROMATIC HYDROCARBONS
SYNTHESIS
VIRUSES