Fluorescence properties of a benzo(a)pyrene 7,8 dihydrodiol 9,10-oxide-DNA adduct. Conformation and effects of intermolecular DNA interactions

Journal Article · Wed Jun 13 00:00:00 EDT 1979 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(79)91476-1· OSTI ID:5927727

Prusik, T ^[1]; Geacintov, N E

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1. New York Univ., NY

The pyrene-like fluorescence of the covalent benzo(a)pyrene diol-epoxide-DNA complex prepared by reacting 7,8,-dihydrodiol 9,19-epoxy benzo(a)pyrene with DNA in aqueous solution in vitro, has been investigated. It is shown that this fluorescence is sensitive to molecular oxygen, to the concentration of native DNA and to the ionic strength, but is insensitive to the concentration of denatured DNA. These effects are related to the conformation of the pyrene-like chromophore of BPDE. Most of the fluorescence of a dilute solution of the DNA-bound benzo(a)pyrene derivative originates from binding sites in which the pyrene moiety is not intercalated between the DNA base pairs, but is located on the outside of the DNA double helix.

• DOE Contract Number: AS02-78EV04959
• OSTI ID: 5927727
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 88:3; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

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550400 -- Genetics
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AROMATICS
BENZOPYRENE
BINDING ENERGY
BIOCHEMICAL REACTION KINETICS
CHEMICAL BONDS
CHEMICAL PROPERTIES
CONDENSED AROMATICS
CONFORMATIONAL CHANGES
DNA
ELEMENTS
EMISSION SPECTROSCOPY
ENERGY
EQUATIONS
FLUORESCENCE SPECTROSCOPY
HYDROCARBONS
INTERACTIONS
INTERMOLECULAR FORCES
KINETICS
NONMETALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN
PAIRING INTERACTIONS
REACTION KINETICS
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS