Specificity of chemiluminescence in the metabolism of benzo(a)pyrene to its carcinogenic diol epoxide

Journal Article · Sun Feb 01 04:00:00 EST 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.78.2.940· OSTI ID:6503517

Hamman, J P; Seliger, H H; Posner, G H

The metabolism of 7,8-dihydroxy-7,8-dihydrobenzo(a)pyrene results primarily in the ultimate carcinogenic metabolite of benzo(a)pyrene, 7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene and to a lesser extent 7,8-dihydroxy-9,10-dioxetane-7,8,9,10-tetrahydrobenzo(a)pyrene, from which chemiluminescence is observed. This specific microsomal chemiluminescence has been used to establish that the rate-limiting reaction in the metabolism of benzo(a)pyrene to the bay region diol epoxide is the production of the 7,8-diol. The microsome-mediated chemiluminescence of the parent benzo(a)pyrene is therefore an indicator of the activity of the specific sequence of metabolic reactions leading to the ultimate carcinogenic metabolite.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 6503517
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:2; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL PATHWAYS
BODY
CELL CONSTITUENTS
CHEMILUMINESCENCE
CONDENSED AROMATICS
DATA
DIGESTIVE SYSTEM
EPOXIDES
EXPERIMENTAL DATA
GLANDS
GLYCOLS
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
LIVER
LUMINESCENCE
MAMMALS
METABOLISM
METABOLITES
MICROSOMES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
RATS
RODENTS
VERTEBRATES