Application of an in vivo mutagenesis system to assess aminothiol effects on neutron-induced genotoxic damage in mouse spleenocytes

Conference · Sat Dec 31 23:00:00 EST 1988

OSTI ID:7244335

Basic, I ^[1]; Grdina, D J; Lyons, T ^[2]

---

1. Zagreb Univ. (Yugoslavia). Dept. of Animal Physiology
2. Argonne National Lab., IL (USA)

A cloning technique has been developed to quantitate and study {ital in vivo} somatic mutations at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in human lymphocytes. In this paper we describe a modification of this assay to quantify HGPRT mutations in mouse spleenocytes. In particular, we have investigated the effects of the aminothiol on mutagenesis induced by single doses of whole body exposures to fission-spectrum neutrons from the JANUS reactor at Argonne National Laboratory. 7 refs., 3 tabs.

• Research Organization: Argonne National Lab., IL (USA)
• Sponsoring Organization: DOE/ER; DOHHS
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 7244335
• Report Number(s): CONF-8910367-1; ON: DE90007804; CNN: CA 37435
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANL
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL ANALYSIS
CLONING
CONNECTIVE TISSUE CELLS
DOSE EQUIVALENTS
ELEMENTARY PARTICLES
ENZYMES
EXTERNAL IRRADIATION
FERMIONS
FISSION NEUTRONS
HADRONS
IN VIVO
IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MUTAGENESIS
MUTATIONS
NATIONAL ORGANIZATIONS
NEUTRONS
NUCLEONS
PHOSPHORUS-GROUP TRANSFERASES
QUANTITATIVE CHEMICAL ANALYSIS
RODENTS
SOMATIC CELLS
SOMATIC MUTATIONS
SPLEEN CELLS
SULFUR COMPOUNDS
TOXICITY
TRANSFERASES
US AEC
US DOE
US ERDA
US ORGANIZATIONS
VERTEBRATES
WHOLE-BODY IRRADIATION