Molecular and clonal analysis of in vivo hprt (hypoxanthine-guanine phosphoribosyl-transferase) mutations in human cells

Conference · Tue Aug 08 04:00:00 EDT 1989

OSTI ID:6708412

Albertini, R J; O'Neill, J P; Nicklas, J A; Allegretta, M ^[1]; Recio, L; Skopek, T R ^[2]

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1. Vermont Univ., Burlington, VT (USA). Genetics Lab.
2. Chemical Industry Inst. of Toxicology, Research Triangle Park, NC (USA)

There is no longer doubt that gene mutations occur in vivo in human somatic cells, and that methods can be developed to detect, quantify and study them. Four assays are now available for such purpose; two detecting mutations that arise in bone marrow erythroid stem cells and two defining mutations that occur in T-lymphocytes. The red cell assays measure changes in mature red blood cells that involve either the blood group glycophorin-A locus or the hemoglobin loci; the lymphocyte assays score for genetic events at either the X-chromosomal hypoxanthine-guanine phosphoribosyl-transferase (hprt) locus. We describe here our attempts in studying in vivo gene mutations in human T-lymphocytes. 35 refs., 3 figs., 3 tabs.

• Research Organization: Vermont Univ., Burlington, VT (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG02-87ER60502
• OSTI ID: 6708412
• Report Number(s): CONF-8907145-2; ON: DE90015561
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300 -- Cytology
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ERYTHROCYTES
GENE MUTATIONS
IN VIVO
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MOLECULAR BIOLOGY
MUTAGENESIS
MUTATIONS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS