Mouse model for somatic mutation at the HPRT (hypoxanthine phosphoribosyl-transferase) gene: Molecular and cellular analyses

Conference · Tue Jul 11 04:00:00 EDT 1989

OSTI ID:5875130

Burkhart-Schultz, K.; Strout, C. L.; Jones, I. M.

Our goal is to use the mouse to model the organismal, cellular and molecular factors that affect somatic mutagenesis in vivo. A fundamental tenet of genetic toxicology is that the principles of mutagenesis identified in one system can be used to predict the principles of mutagenesis in another system. The validity of this tenet depends upon the comparability of the systems involved. To begin to achieve an understanding of somatic mutagenesis in vivo, we have been studying mutations that occur in the hypoxanthine phosphoribosyl-transferase (HPRT) gene of lymphocytes of mice. Our in vivo model for somatic mutation allows us to analyse factors that affect somatic mutation. Having chosen the mouse, we are working with cells in which the karyotype is normal, and metabolic and DNA repair capacity are defined by the mouse strain chosen. At the organismal level, we can vary sex, age, the exposure history, and the tissue source of cells analysed. (All studies reported here have, however, used male mice.) At the cellular level, T lymphocytes and their precursors are the targets and reporters of mutation. 26 refs., 1 fig., 1 tab.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5875130
• Report Number(s): UCRL-101498; CONF-8907113-6; ON: DE89015334
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
DISEASES
DNA HYBRIDIZATION
DNA REPAIR
DNA-CLONING
ENZYMES
GENE MUTATIONS
GLYCOSYL TRANSFERASES
HYBRIDIZATION
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
METABOLIC DISEASES
MICE
MUTAGEN SCREENING
MUTATION FREQUENCY
MUTATIONS
PENTOSYL TRANSFERASES
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
SCREENING
SOMATIC CELLS
TRANSFERASES
VERTEBRATES