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The development of in vitro mutagenicity testing systems using T-lymphocytes

Technical Report ·
DOI:https://doi.org/10.2172/7049865· OSTI ID:7049865

This work has focused on the development of in vitro T-cell mutation assays. Conditions have been defined to measure the in vitro induction of mutations at the hypoxanthine-guanine phosphoribosyl transferase (hprt) locus in human T-lymphocytes. This assay is a parallel to our in vivo hprt assay, in that the same cells are utilized. However, the in vitro assay allows for carefully controlled dose response studies. 21 refs., 16 figs., 13 tabs.

Research Organization:
Vermont Univ., Burlington, VT (United States)
Sponsoring Organization:
DOE; USDOE, Washington, DC (United States)
DOE Contract Number:
FG02-87ER60502
OSTI ID:
7049865
Report Number(s):
DOE/ER/60502-6; ON: DE92015918
Country of Publication:
United States
Language:
English