Isolation and characterization of 14 CA-repeat microsatellites from human chromosome 21

Journal Article · Fri Oct 01 00:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1442· OSTI ID:7200760

Bosch, A; Nunes, V; Estivill, X ^[1]; Patterson, D ^[2]

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1. Cancer Research Institute, Barcelona (Spain)
2. Eleanor Roosevelt Institute for Cancer Research, Denver, CO (United States)

Fourteen CA-repeat microsatellite polymorphisms from human chromosome 21 (HC21) (D21S364, D21S365, D21S366, D21S367, D21S368, D21S369, D21S406, D21S407, D21S408, D21S409, D21S410, D21S411, D21S415, and D21S1217) have been cloned from a flow-sorted phage library (LA21NS01). These microsatellite loci have been mapped using a somatic cell hybrid panel of HC21, and heterozygosities and allele frequencies have been determined. The markers spread along the length of the long arm of the HC21. Twelve of these microsatellites have heterozygosities between 0.47 and 0.90. These new CA-repeat markers will improve the genetic map of HC21. 13 refs., 1 fig., 1 tab.

• OSTI ID: 7200760
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 18:1; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
GENETIC MAPPING
HUMAN CHROMOSOME 21
HUMAN CHROMOSOMES
MAPPING
RFLPS