Microsatellite polymorphism linkage map of human chromosome 13q

Journal Article · Mon Feb 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1071· OSTI ID:6660259

Bowcock, A; Osborne-Lawrence, S; Barnes, R; Dunn, C ^[1]; Chakravarti, A; Washington, S ^[2]

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1. Univ. of Texas Southwestern Center, Dallas (United States)
2. Univ. of Pittsburgh, PA (United States)

Twelve polymorphic (CA)[sub n] microsatellites were isolated from a flow-sorted chromosome 13 genomic library. These, and two others that have been previously described, were genotyped in 41 families from the CEPH (Centre d'Etude Polomorphisme Humain, Paris) and a primary linkage map with considerable support for order (odds > 10,000:1) was constructed. Two RFLP-based markers, COL4A1 and D13S52, with heterozygosities above 0.67 and an RFLP-based centromeric marker at D13Z1 were include in this map which extend from 13cen to 13q34. The heterozygosity of all of the PCR-based markers is above 60%. The total map spans a genetic distance of 144 cM, extending from D13Z1 to D13S52 with a single maximum intermarker recombination distanct of 35 cM. All other intermarker recombination distance are 18 cM or less. Marker order was confirmed by sublocalizing many of the microsatellite-containing clones on a panel of rodent-human somatic cell hybrids with deletions and rearrangements of chromosome 13. One spontaneous new mutation for these 14 (CA)[sub n] repeat markers was identified from a total of 8006 gametes, giving an overall observed spontaneous mutation rate of 0.00012 per locus per gamete. An integrated map of chromosome 13q was constructed with the microsatellite markers described here and previously genotyped RFLP-based markers. This sex-average map spans 209 cM with an average distance between unique map locations of 4.5 cM; the maximum intermarker distance was 14 cM. 35 refs., 3 figs., 4 tabs.

• OSTI ID: 6660259
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
DISTANCE
GAMETES
GENETIC MAPPING
GERM CELLS
HUMAN CHROMOSOMES
MAPPING
RECOMBINATION
RFLPS