Index, microsatellite and comprehensive linkage maps of human chromosome 14 with cytogenetic tie points
- Washington Univ., School of Medicine, St. Louis, MO (United States); and others
We have constructed an index linkage map of human chromosome 14 which consists of 16 markers with heterozygosities of at least 68% and intermarker spacing of no greater than 16 cM. Our map was constructed using the program package CRI-MAP and the genotypic data from the primary (40 family) CEPH pedigree resource. All of the index markers are PCR-based microsatellite polymorphisms with heterozygosities that range from 68-91% (average 78%). The sex-average length of the index map is 118 cM and includes all markers uniquely placed with odds of at least 1000:1. The female map is 142 cM in length and the male map is 97 cM. The 14q telomere is anchored by the microsatellite marker SCAW1R. Hence, our map which includes this marker extends to the physical terminus of the long arm of chromosome 14. We have also constructed two other maps, namely a microsatellite map and a comprehensive map. The microsatellite map includes all the previously described microsatellites. 33 of these microsatellite markers have been genotyped through the entire 40 CEPH family panel. Of the 48 loci used to construct the microsatellite map, 36 loci were uniquely placed with odds greater than 1000:1. The sex-average length of the map is 121 cM with an average intermarker distance of 3.36 cM. The comprehensive map was built with 72 loci (85 systems) and includes all of the microsatellites from the microsatellite map and all of the RFLP markers from the baseline map. The sex-average length of the comprehensive map is 137 cM with 45 loci uniquely placed. We have also isolated YACs corresponding to six loci. Alu-PCR products from these YACs were used for fluorescence in-situ hybridizations. By physically localizing polymorphic markers, our maps provide six new correlations (10 out of a total of 13 bands correlated at 550 band resolution) between the linkage and cytogenetic maps and further map integration efforts for this chromosome.
- OSTI ID:
- 134000
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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