Searching for new microsatellite markers in the genetic gaps of human Chromosome 21
- Hospital Duran i Reynals, Catalunya (Spain); and others
The genetic map of human chromosome 21 (HC21) has been vastly improved during the last few years, but there are still several gaps with few highly informative markers available. To accomplish the saturation of the HC21 map, markers are being developed from YAC contigs of these regions. We have analyzed 10 YAC clones from the Genethon HC21 YAC panel using PCR with Alu and CA/GT primers. The PCR products were subcloned, sequenced and one flanking primer designed. To obtain the remaining flanking primer we used various methods such as vectorette PCR, nested and inverse PCR. In some cases a lambda phage genomic library was screened with the specific primer to obtain the opposite CA-repeat flanking sequence. Several microsatellites have been isolated from each YAC with heterozygosities greater than 0.50 and they are being characterized for inclusion in the genetic map of HC21. The isolation of these new markers is also useful for the characterization of YAC clones, for instance for physical mapping.
- OSTI ID:
- 134691
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-1429
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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