Targeted development of microsatellite markers from inter-Alu amplification of YAC clones

Journal Article · Fri Jan 14 23:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1079· OSTI ID:7076710

De Souza, A P ^[1]; Allamand, V ^[2]; Richard, I; Brenguier, L ^[3]; Chumakov, I; Cohen, D; Beckmann, J S ^[4]

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1. Universidade Estadual de Campinas (Brazil) Genethon, Evry (France)
2. Genethon, Evry (France) Foundation Jean Dausset/CEPH, Paris (France)
3. Genethon, Evry (France)
4. Foundation Jean Dausset/CEPH, Paris (France)

Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers achieved by selectively cloning and sequencing (CA)[sub n]-positive human inter-Alu sequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers. 11 refs., 1 fig., 1 tab.

• OSTI ID: 7076710
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:2; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENETIC MAPPING
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
STRUCTURAL CHEMICAL ANALYSIS