Integrated map of new CA-repeats of human chromosome 21
- Hospital Duran i Reynals, Catalunya (Spain)
- Eleanor Roosevelt Institute, Denver, CO (United States); and others
To contribute to the map of human chromosome 21 (HC21), 35 new CA-repeats isolated from an HC21 phage library (LA21NS01) have been characterized and localized on HC21 using somatic cell hybrids and the Genethon YAC panel. Their heterozygosities range between 0.10 and 0.90 with an average of 0.70. Twenty of them, with heterozygosities between 0.47 and 0.83, have been analyzed in the CEPH reference families. A linkage map was constructed using the CRI-MAP program with 46 markers. This linkage map contains 22 uniquely placed loci, ordered with odds of at least 1000:1, with an average size interval of 3.5 cM. 10 markers were approximately positioned on the map. The sex average length of the map is 73.4 cM, with male and female lengths being 64.2 and 83.5 cM, respectively. D21S1262 was placed in the same megalocus as the IFNAR and GART genes. D21S369 seems to be the most pericentromeric microsatellite marker on HC21 based on linkage analysis, with a heterozygosity of 0.60, and may be very useful for nondisjunction studies. Integration of the somatic cell hybrid, TAC and/or linkage maps have provided a more defined location for these 35 new CA-repeat markers which will contribute greatly to the integrated map of HC21.
- OSTI ID:
- 134682
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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