Human platelet glycoprotein IX: An adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.17.6773· OSTI ID:7195137

Hickey, M J; Williams, S A; Roth, G J ^[1]

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1. Seattle Veterans Administration Medical Center, Seattle, WA (USA)

The glycoprotein (GP) Ib-IX complex on the surface of human platelets functions as the von Willebrand factor receptor and mediates von Willebrand factor-dependent platelet adhesion to blood vessels. GPIX is a relatively small (M{sub r}, 17,000) protein that may provide for membrane insertion and orientation of the larger component of the complex. GPIb (M{sub r}, 165,000). Using antibody screening, the authors cloned a cDNA encoding GPIX from a human erythroleukemia cell cDNA library constructed in phage {lambda}gt11. Lacking a 5{prime} untranslated region and start codon, the cDNA sequence includes 604 nucleotides, beginning with 495 bases at the 5{prime} end coding for 165 amino acids, followed by a stop codon and 106 noncoding bases at the 3{prime} end. By Northern blot analysis, the GPIX cDNA hybridizes with a single 1.0-kilobase species of platelet poly(A){sup +} RNA. Translation of the cDNA sequence gives a predicted protein sequence beginning with a truncated putative signal sequence of 5 amino acids followed by a sequence of 17 amino acids matching that determined directly by Edman degradation of intact GPIX. GPIX contains a leucine-rich glycoprotein (LRG) sequence of 24 amino acids similar to conserved LRG sequences in GPIb and other proteins from humans, Drosophila, and yeast. The role of the flank-LRG center-flank structure in the evolution and function of the LRG proteins remains to be defined.

• OSTI ID: 7195137
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:17; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ALKALI METAL COMPOUNDS
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA SEQUENCING
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLYCOPROTEINS
HALIDES
HALOGEN COMPOUNDS
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LEUCINE
LEUKEMIA
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
METHIONINE
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOSPHORS
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
SODIUM COMPOUNDS
SODIUM IODIDES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
TUMOR CELLS