Organization of the gene for platelet glycoprotein IIb

Journal Article · Tue Feb 06 04:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00457a020· OSTI ID:7064507

Heidenreich, R ^[1]; Eisman, R; Surrey, S; Delgrosso, K; Schwartz, E; Poncz, M ^[2]; Bennett, J S ^[3]

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1. Children's Hospital of Philadelphia, PA (USA)
2. Children's Hospital of Philadelphia, PA (USA) Univ. of Pennsylvania, Philadelphia (USA)
3. Hospital of the Univ. of Pennsylvania, Philadelphia (USA) Univ. of Pennsylvania, Philadelphia (USA)

The glycoprotein (GP) IIb/IIIa heterodimer functions as a receptor for fibrinogen, von Willebrand factor, and fibronectin on activated platelets; it is dysfunctional in the bleeding diathesis Glanzmann's thrombasthenia. This receptor is a member of the integrin family, which includes homologous membrane receptors involved in a number of different cell-cell and cell-matrix adhesive interactions. Knowledge of the sequence and organization of the GPIIb and GPIIIa genes will help in understanding evolutionary relationships and functional homologies of this family of adhesion protein receptors and will facilitate analysis of molecular defects responsible for thrombasthenia. Using the GPIIb cDNA as a probe, the authors have isolated overlapping genomic clones encompassing the entire coding region, the 5{prime}- and 3{prime}-untranslated sequences, and the immediate flanking regions for the GPIIb gene. The gene spans approximately 17.2 kilobases (kb); all but approximately 2.6 kb of intronic DNA sequence has been determined. The GPIIb gene contains 30 exons whose demarcations do not correlate with previously suggested functional domains. Two intron/exon borders have the rare GC splice donor sequence instead of the consensus GT sequence. There are at least seven complete and three partial AluI sequence repeats within the intron sequences. The immediate 5{prime}-flanking sequence of rodent GPIIb demonstrates complete identity near the proposed cap site with its human counterpart, but again, no TATA or CAAT boxes are apparent.

• OSTI ID: 7064507
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:5; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
COAGULANTS
DNA
DNA SEQUENCING
DRUGS
FIBRINOGEN
GENES
GLOBULINS
GLYCOPROTEINS
HEMATOLOGIC AGENTS
HEMOSTATICS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RECEPTORS
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES