The structure and complete nucleotide sequence of the human cyclophilin 40 (PPID) gene

Journal Article · Thu Aug 01 00:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0384· OSTI ID:465979

Yokoi, Haruhiko; Shimizu, Yukiko; Anazawa, Hideharu ^[1]

---

1. Tokyo Research Labs., Machida (Japan); and others

Cyclophilin 40 is a recently identified member of the cyclophilin family that is found in an unactivated steroid hormone receptor complex. Cyclophilin 40 possesses a region homologous to FKBP59, a member of the FK506-binding protein family that is also a component of the receptor complex. We report the isolation and sequencing of the entire human cyclophilin 40 (hCyP40) gene (human gene symbol PPID). The gene contains 10 exons (43 to 698 bp) and 9 introns encompassing 14.2 kb. The exon organization of the cyclophilin-like region is not similar to that of the human cyclophilin A gene (PPIA), suggesting their early divergence in evolution. Determination of the sequence of the 5{prime} end of the hCyP40 mRNA by an {open_quotes}anchor-ligation PCR{close_quotes} procedure showed that transcription is initiated from a cluster of sites about 80 bp upstream from the first in-frame ATG. The immediate 5{prime}-flanking region of the gene lacks typical TATA and CAAT boxes, but is GC-rich and contains Sp1 sites, features characteristic of promoters associated with housekeeping genes. The hCyP40 gene was mapped to chromosome 4 by PCR with genomic DNA from somatic cell hybrids. As shown by {open_quotes}Zoo blot{close_quotes} analysis, the cylophilin 40 gene appears to be highly conserved throughout evolution. 47 refs., 4 figs., 1 tab.

• OSTI ID: 465979
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 35; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL EFFECTS
BIOLOGICAL EVOLUTION
DNA SEQUENCING
DNA-CLONING
GENE REGULATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOMES
HYBRIDIZATION
IMMUNOSUPPRESSIVE DRUGS
NUCLEOTIDES
POLYMERASE CHAIN REACTION
PROTEINS
RECEPTORS
SOMATIC CELLS
STEROID HORMONES
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION