The. alpha. and. beta. chains of human platelet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.7.2135· OSTI ID:6897468

Lopez, J A; Chung, D W; Fujikawa, Kazuo; Davie, E W ^[1]; Hagen, F S ^[2]; Roth, G J ^[3]

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1. Univ. of Washington, Seattle (USA)
2. ZymoGenetics, Inc., Seattle, WA (USA)
3. Univ. of Washington, Seattle (USA) Veterans Administration Medical Center, Seattle, WA (USA)

The primary structure of the {beta} chain of human glycoprotein Ib (GPIb), the platelet receptor for von Willebrand factor, has been established by a combination of cDNA cloning and amino acid sequence analysis. A {lambda} phage cDNA expression library prepared from human erythroleukemia cells (HEL cells) was screened with a radiolabeled affinity-purified rabbit polyclonal antibody to the {beta} chain of GPIb. Eighteen positive clones were isolated and plaque-purified and the nucleotide sequences of three were determined. The composite sequence spanned 968 nucleotides and included a 5{prime} untranslated region of 22 nucleotides, an open reading frame of 618 nucleotides encoding a signal peptide of 28 amino acids and a mature protein of 181 amino acids, a stop codon, and a 3{prime} noncoding region of 307 nucleotides. The 3{prime} noncoding sequence also contained a polyadenylylation signal (AATAAA) 14 nucleotides upstream from the poly(A) tail of 18 nucleotides. Edman degradation of the intact {beta} chain and of peptides produced by chemical cleavage yielded amino acid sequences spanning 76 residues that were identical to those predicted from the cDNA. The amino-terminal region of the {beta} chain contains a leucine-rich sequence of 24 amino acids that is similar to a sequence that occurs as seven tandem repeats in the {alpha} chain of GPIb and nine tandem repeats in leucine-rich {alpha}{sub 2}-glycoprotein. The amino-terminal region of the {beta} chain of GPIb is followed by a transmembrane segment of 25 amino acids and an intracellular segment of 34 amino acids at the carboxyl terminus of the protein.

• OSTI ID: 6897468
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:7; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENS
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ESTERS
EVEN-ODD NUCLEI
GENE REGULATION
GENES
GLUCOPROTEINS
GLYCOPROTEINS
HALIDES
HALOGEN COMPOUNDS
HEMIC DISEASES
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LEUCINE
LEUKEMIA
LIGHT NUCLEI
MATERIALS
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
PHORBOL ESTERS
PHOSPHORS
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
SACCHARIDES
SODIUM COMPOUNDS
SODIUM IODIDES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
TUMOR CELLS