Cloning of the. cap alpha. chain of human platelet glycoprotein Ib: a transmembrane protein with homology to leucine-rich. cap alpha. /sub 2/-glycoprotein

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.16.5615· OSTI ID:5175280

Lopez, J A; Chung, D W; Fujikawa, K; Hagen, F S; Papayannopoulou, T; Roth, G J

Glycoprotein Ib is a surface membrane glycoprotein of platelets that functions as a receptor for von Willebrand factor. It is a heterodimer composed of an ..cap alpha.. and a ..beta.. chain linked by a disulfide bond(s). A phage lambdagt11 cDNA expression library prepared from mRNA from a human erythroleukemia cell line, HEL, was screened using an affinity-purified antibody to the glycocalicin portion of the ..cap alpha.. chain of a glycoprotein Ib. Eleven positive clones were isolated and plaque-purified. The largest cDNA insert was 2420 nucleotides in length and coded for a leader sequence of 16 amino acids, a mature protein of 610 amino acids, and a stop codon. It also contained 42 nucleotides of 5' noncoding sequence and 497 nucleotides of 3' noncoding sequence, including a poly(A) tail. The amino acid sequence of the ..cap alpha.. chain of GPIb predicted from the cDNA agreed completely with the sequence of 156 amino acids that was determined by Edman degradation of peptides isolated from human platelet glycocalicin after digestion with trypsin or Staphylococcus aureus V8 protease. The extracytoplasmic domain of the ..cap alpha.. subunit of GPIb contains several noteworthy structural features, including a region of seven tandem repeats of 24 amino acids that are homologous with those present in leucine-rich ..cap alpha../sub 2/-glycoprotein. The extracytoplasmic domain also contains two hydrophilic regions, one rich in charged amino acids and a second rich in serine and threonine resides. The extracytoplasmic domain is followed by a potential transmembrane segment of approximately 29 amino acids and a potential intracellular domain of approximately 200 amino acids located at the carboxyl end of the molecule.

• Research Organization: Univ. of Washington, Seattle (USA)
• OSTI ID: 5175280
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:16; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ADHESION
ALKALI METAL COMPOUNDS
AMINO ACID SEQUENCE
AMINO ACIDS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENS
CELL CONSTITUENTS
CELL MEMBRANES
CLONING
COAGULANTS
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DNA-CLONING
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
EVEN-ODD NUCLEI
GENETIC MAPPING
GLUCOPROTEINS
HALIDES
HALOGEN COMPOUNDS
HEMATOLOGIC AGENTS
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LEUCINE
LIGHT NUCLEI
MAPPING
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORS
PROTEINS
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
RNA
SACCHARIDES
SODIUM COMPOUNDS
SODIUM IODIDES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES