Regeneration of blood-forming organs after autologous leukocyte transfusion in lethally irradiated dogs. II. Distribution and cellularity of the marrow in irradiated and transfused animals. [X radiation]
Dogs were given transfusions of cryopreserved autologous mononuclear blood leukocytes after 1200 roentgens (R) (midline dose) whole-body x-irradiation. Bone marrow repopulation was studied by means of histomorphological methods at days 9 and 10 after transfusion of an average of 3 x 10/sup 9/, 7 x 10/sup 9/, 13 x 10/sup 9/, and 31 x 10/sup 9/ cells. The return of marrow cellularity to normal values was related to the number of cells transfused. With low cell doses (3 x 10/sup 9/ and 7 x 10/sup 9/), the marrow regeneration at 10 days was focal. There were groups of cells (colonies) showing either erythropoiesis, myelopoiesis, or megakaryocytopoiesis in the osteal niches of the trabecular bones. Frequently such niches were seen showing complete cellular recovery next to niches with complete aplasia. With higher cell doses, all niches showed hemopoietic regeneration, and the cellularity approached normal values. No hemopoietic regeneration was observed in those skeletal parts that do not show hemopoiesis, even under normal circumstances.
- Research Organization:
- Univ. of Ulm, Ger.
- OSTI ID:
- 7193001
- Journal Information:
- Blood; (United States), Vol. 47:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW
BIOLOGICAL REGENERATION
HEMATOPOIETIC SYSTEM
BIOLOGICAL RADIATION EFFECTS
LEUKOCYTES
TRANSFUSIONS
BLOOD FORMATION
DOGS
LETHAL IRRADIATION
X RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
ORGANS
RADIATION EFFECTS
RADIATIONS
RECOVERY
THERAPY
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals