Repopulation of lymph nodes of dogs after 1200 R whole-body x-irradiation and intravenous administration of mononuclear blood leukocytes

Journal Article · Sun Aug 01 00:00:00 EDT 1976 · Am. J. Pathol.; (United States)

OSTI ID:7127916

Nelson, B; Calvo, W; Fliedner, T M; Herbst, E; Bruch, C; Schnappauf, H P; Flad, H D

Fresh and cryopreserved autologous or allogeneic mononuclear blood cells (MBCs) intravenously injected in 1200 R total-body x-irradiated dogs repopulated lymph nodes within 10 days after transfusion. Several parameters of the lymphopoietic regeneration were correlated with the number of cells transfused and with the number of colony-forming units contained in the cell suspension when they were cultured in agar (CFU/sub c/). Values within the normal or close to normal range were reached in the mesenteric nodes of most of the animals transfused with 10 x 10/sup 9/ MBC or more. These values were obtained when 5 x 10/sup 5/ CFU/sub c/ or more were transfused. Axillary nodes showed lower values than mesenteric nodes. They were mostly under the normal range but well over those of the irradiated controls. Frozen and thawed MBCs seem to be as effective as fresh cells for lymphopoietic restoration. The mesenteric nodes of dogs transfused with allogeneic MBCs showed higher cellularity and larger cortical-paracortical areas than those of dogs transfused with approximately the same number of autologous cells. The repopulation of lymph nodes parallels that of the marrow.

• Research Organization: Univ. of Ulm, Germany
• OSTI ID: 7127916
• Journal Information: Am. J. Pathol.; (United States), Vol. 84:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BLOOD FORMATION
BIOLOGICAL RADIATION EFFECTS
LYMPH NODES
BIOLOGICAL REGENERATION
CELL PROLIFERATION
CFU
DOGS
LEUKOCYTES
X RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY FLUIDS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LYMPHATIC SYSTEM
MAMMALS
RADIATION EFFECTS
RADIATIONS
RECOVERY
VERTEBRATES
560152* - Radiation Effects on Animals- Animals