Cytogenetic studies in dogs after total body irradiation and allogeneic transfusion with cryopreserved blood mononuclear cells: observations in long-term chimeras

Journal Article · Thu Mar 01 04:00:00 EST 1984 · Int. J. Cell Cloning; (United States)

DOI:https://doi.org/10.1002/stem.5530020202· OSTI ID:7004230

Carbonell, F; Calvo, W; Fliedner, T M; Kratt, E; Gerhartz, H; Koerbling, M; Nothdurft, W; Ross, W M

Cytogenetic studies were performed on two dog groups after total body irradiation and allogeneic transfusion with cryopreserved blood mononuclear cells. The first group of dogs was transfused with unseparated leukocytes and suffered from graft-versus-host disease (GvHD). Cytogenetic studies demonstrated only cells of donor origin in all dogs of this group. The second group of animals was transfused with fraction 2 of a discontinuous albumin gradient. The dogs of this group did not develop GvHD, and the cytogenetic studies showed the presence of a mosaic of cells from donor and recipient origin in all of them. These results suggest that the GvHD may suppress autochthonous regeneration.

• Research Organization: Department of Clinical Physiology and Occupational Medicine, University of Ulm, West Germany
• OSTI ID: 7004230
• Journal Information: Int. J. Cell Cloning; (United States), Journal Name: Int. J. Cell Cloning; (United States) Vol. 2:2; ISSN IJCCE
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BODY FLUIDS
CRYOBIOLOGY
CYTOLOGY
DOGS
EXTERNAL IRRADIATION
GRAFT-HOST REACTION
IRRADIATION
MAMMALS
MATERIALS
THERAPY
TRANSFUSIONS
VERTEBRATES
WHOLE-BODY IRRADIATION