Rapid, solid phase radioimmunoassay for prostaglandin F/sub 2/. cap alpha. and its main circulating metabolite. [Tritium tracer technique]

Journal Article · Fri May 21 04:00:00 EDT 1976 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(76)90155-X· OSTI ID:7184689

Fitzpatrick, F A; Wynada, M A

Antibodies directed against prostaglandin F/sub 2/..cap alpha.. and its main circulating metabolite, 13,14-dihydro-15-keto-prostaglandin F/sub 2/..cap alpha.., were insolubilized by reaction with ethylchloroformate. Suspensions of insolubilized antibodies retained immunogenic activity and were useful for the development of a simple and rapid solid phase radioimmunoassay for these two prostaglandins. Using this method, suppression of prostaglandin levels by the influence of indomethacin in vivo has been observed. Advantages of solid phase radioimmunoassay over current procedures are discussed.

• Research Organization: The Upjohn Co., Kalamazoo, MI
• OSTI ID: 7184689
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 73:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-EVEN NUCLEI
PROSTAGLANDINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES