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Improved sensitivity of iodinated histamine: prostaglandin radioimmunoassay by prostaglandin methyl esters

Journal Article · · Anal. Biochem.; (United States)
; ; ;
Use of (/sup 125/I)-labeled histamine-prostaglandin tracer increases the sensitivity of the radioimmunoassays of prostaglandin derivatives. Six different antisera were produced for prostaglandins and their derivatives (prostaglandins E/sub 1/, E/sub 2/, F/sub 1..cap alpha../, F/sub 2..cap alpha../, 13,14-dihydro-15-ketoprostaglandin E/sub 2/, and 13,14-dihydro-15-ketoprostaglandin F/sub 2..cap alpha../) and were investigated with the corresponding tritiated and iodinated tracers. Displacement of iodinated tracers by the methyl esters of the prostaglandin compounds resulted, in most cases, in a three- to fivefold increase in sensitivity compared to unesterified inhibitors. Esterification also caused some alteration in the specificities observed. Our results suggest that conformational changes in the esterified prostaglandins (tracer and inhibitor) could explain these charges.
Research Organization:
Institut Pasteur, Paris
OSTI ID:
6934680
Journal Information:
Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 87:1; ISSN ANBCA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
AZOLES
BETA DECAY RADIOISOTOPES
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ESTERIFICATION
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROSTAGLANDINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SENSITIVITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS